Caenorhabditis elegans chromosome arms are anchored to the nuclear membrane via discontinuous association with LEM-2.
Published in Genome Biology
Methods for identifying Neisseria meningitidis carriers: a multi-center study in the African meningitis belt.
...Published in PLoS ONE
An outbreak of meningococcal disease in Gloucestershire.
Published in The Lancet
Between October, 1981, and March, 1986, there were 65 cases of meningococcal infection, about five times the expected number, in Gloucester Health District. The cases, mainly in teenagers and young adults, were clustered in the Stroud district and in the southern part of Gloucester City, and most were caused by a sulphonamide-resistant group B type...
DUPCAR: reconstructing contiguous ancestral regions with duplications.
Published in Journal of Computational Biology
Accurately reconstructing the large-scale gene order in an ancestral genome is a critical step to better understand genome evolution. In this paper, we propose a heuristic algorithm, called DUPCAR, for reconstructing ancestral genomic orders with duplications. The method starts from the order of genes in modern genomes and predicts predecessor and ...
Imaging defect formation in the template growth of NiSi2/Si(111): An application of quantum size microscopy.
Published in Physical Review B
Prevention of meningococcal infection in laboratory workers—an audit of practice in England and Wales.
Published in Communicable disease and public health / PHLS
A survey of clinical microbiology laboratories in England and Wales found that in 12% of laboratories a class 1 microbiological safety cabinet was not always used for manipulating suspensions of live meningococci. The recommendation that a safety cabinet should always be used for such purposes needs reiteration and implementation.
Caenorhabditis elegans polo-like kinase PLK-1 is required for merging parental genomes into a single nucleus.
Published in Molecular Biology of the Cell
Before the first zygotic division, the nuclear envelopes of the maternal and paternal pronuclei disassemble, allowing both sets of chromosomes to be incorporated into a single nucleus in daughter cells after mitosis. We found that in Caenorhabditis elegans, partial inactivation of the polo-like kinase PLK-1 causes the formation of two nuclei, conta...
Peptide ligand structure and I-Aq binding avidity influence T cell signaling pathway utilization.
Published in Clinical Immunology
Factors that drive T cells to signal through differing pathways remain unclear. We have shown that an altered peptide ligand (A9) activates T cells to utilize an alternate signaling pathway which is dependent upon FcRγ and Syk. However, it remains unknown whether the affinity of peptide binding to MHC drives this selection. To answer this question ...
Functional sites of interaction between release factor RF1 and the ribosome.
Published in Nautre Structural Biology
Translational release factors decipher stop codons in mRNA and activate hydrolysis of peptidyl-tRNA in the ribosome during translation termination. The mechanisms of these fundamental processes are unknown. Here we have mapped the interaction of bacterial release factor RF1 with the ribosome by directed hydroxyl radical probing. These experiments i...
The infinite sites model of genome evolution.
Published in Proceedings of the National Academy of Sciences
We formalize the problem of recovering the evolutionary history of a set of genomes that are related to an unseen common ancestor genome by operations of speciation, deletion, insertion, duplication, and rearrangement of segments of bases. The problem is examined in the limit as the number of bases in each genome goes to infinity. In this limit, th...
