Million-Weaver, S Alexander, Dl Allen, Jm Manel Camps
Published in
Methods in Molecular Biology
Our laboratory specializes in directed protein evolution, i.e., evolution of proteins under defined selective pressures in the laboratory. Our target genes are encoded in ColE1 plasmids to facilitate the generation of libraries in vivo. We have observed that when random mutations are not restricted to the coding sequence of the target genes, direct...
Manel Camps Loeb, La
Published in
Methods in Molecular Biology
Alexander, Dl Lilly, J Hernandez, J Romsdahl, J Troll, Cj Manel Camps
Published in
Methods in Molecular Biology
Directed evolution is an approach that mimics natural evolution in the laboratory with the goal of modifying existing enzymatic activities or of generating new ones. The identification of mutants with desired properties involves the generation of genetic diversity coupled with a functional selection or screen. Genetic diversity can be generated usi...
Manel Camps Loeb, La
Published in
Methods in Molecular Biology
Karolchik, D Bejerano, G Hinrichs, As Kuhn, Rm Miller, W Rosenbloom, Kr Zweig, As David Haussler Kent, Wj
Published in
Methods in Molecular Biology
Comparative analysis of DNA sequence from multiple species can provide insights into the function and evolutionary processes that shape genomes. The University of California Santa Cruz (UCSC) Genome Bioinformatics group has developed several tools and methodologies in its study of comparative genomics, many of which have been incorporated into the ...
Blanchette, M Diallo, Ab Green, Ed Miller, W David Haussler
Published in
Methods in Molecular Biology
This chapter introduces the problem of ancestral sequence reconstruction: given a set of extant orthologous DNA genomic sequences (or even whole-genomes), together with a phylogenetic tree relating these sequences, predict the DNA sequence of all ancestral species in the tree. Blanchette et al. (1) have shown that for certain sets of species (in pa...
Manel Camps Loeb, La
Published in
Methods in Molecular Biology
Blanchette, M Diallo, Ab Ed Green Miller, W David Haussler
Published in
Methods in Molecular Biology
This chapter introduces the problem of ancestral sequence reconstruction: given a set of extant orthologous DNA genomic sequences (or even whole-genomes), together with a phylogenetic tree relating these sequences, predict the DNA sequence of all ancestral species in the tree. Blanchette et al. (1) have shown that for certain sets of species (in pa...
Blanchette, M Diallo, Ab Green, Ed Miller, W David Haussler
Published in
Methods in Molecular Biology
This chapter introduces the problem of ancestral sequence reconstruction: given a set of extant orthologous DNA genomic sequences (or even whole-genomes), together with a phylogenetic tree relating these sequences, predict the DNA sequence of all ancestral species in the tree. Blanchette et al. (1) have shown that for certain sets of species (in pa...
David Haussler Miller, W Rosenbloom, K Taylor, J Schaeffer, S Kent, J
Published in
Methods in Molecular Biology
The UC Santa Cruz Genome Browser provides a number of resources that can be used for phylogenomic studies, including (1) whole-genome sequence data from a number of vertebrate species, (2) pairwise alignments of the human genome sequence to a number of other vertebrate genome, (3) a simultaneous alignment of 17 vertebrate genomes (most of them inco...
