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Whole-transcriptome RNAseq analysis from minute amount of total RNA. PDF available through Get Fulltext Research

Tariq, Muhammad A Kim, Hyunsung J Jejelowo, Olufisayo Pourmand, Nader

Published in Nucleic acids research

RNA sequencing approaches to transcriptome analysis require a large amount of input total RNA to yield sufficient mRNA using either poly-A selection or depletion of rRNA. This feature makes it difficult to miniaturize transcriptome analysis for greater efficiency. To address this challenge, we devised and validated a simple procedure for the prepar...

The UCSC Genome Browser database: extensions and updates 2013. PDF available through Get Fulltext Research

Meyer, Laurence R Zweig, Ann S Hinrichs, Angie S Karolchik, Donna Kuhn, Robert M Wong, Matthew Sloan, Cricket A Rosenbloom, Kate R Roe, Greg Rhead, Brooke ...

Published in Nucleic acids research

The University of California Santa Cruz (UCSC) Genome Browser (http://genome.ucsc.edu) offers online public access to a growing database of genomic sequence and annotations for a wide variety of organisms. The Browser is an integrated tool set for visualizing, comparing, analysing and sharing both publicly available and user-generated genomic datas...

Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectros... PDF available through Get Fulltext Research

Long, Xi Parks, Joseph W Bagshaw, Clive R Stone, Michael D

Published in Nucleic acids research

Single-molecule techniques facilitate analysis of mechanical transitions within nucleic acids and proteins. Here, we describe an integrated fluorescence and magnetic tweezers instrument that permits detection of nanometer-scale DNA structural rearrangements together with the application of a wide range of stretching forces to individual DNA molecul...

The UCSC Genome Browser database: update 2011. PDF available through Get Fulltext Research

Fujita, Pauline A Rhead, Brooke Zweig, Ann S Hinrichs, Angie S Karolchik, Donna Cline, Melissa S Goldman, Mary Barber, Galt P Clawson, Hiram Coelho, Antonio ...

Published in Nucleic acids research

The University of California, Santa Cruz Genome Browser (http://genome.ucsc.edu) offers online access to a database of genomic sequence and annotation data for a wide variety of organisms. The Browser also has many tools for visualizing, comparing and analyzing both publicly available and user-generated genomic data sets, aligning sequences and upl...

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