Eason, Rg Nader Pourmand Tongprasit, W Herman, Zs Anthony, K Jejelowo, O Davis, Rw Stolc, V
Published in
Proceedings of the National Academy of Sciences
Incorporation of strain-specific synthetic DNA tags into yeast Saccharomyces cerevisiae gene-deletion strains has enabled identification of gene functions by massively parallel growth rate analysis. However, it is important to confirm the sequences of these tags, because mutations introduced during construction could lead to significant errors in h...
Korostelev, A Trakhanov, S Asahara, H Laurberg, M Lancaster, L Harry Noller
Published in
Proceedings of the National Academy of Sciences
The crystal structure of an initiation-like 70S ribosome complex containing an 8-bp Shine-Dalgarno (SD) helix was determined at 3.8-A resolution. Translation-libration-screw analysis showed that the inherent anisotropic motions of the SD helix were biased along its helical axis, suggesting that during the first step of translocation, the SD helix m...
Fitnat Yildiz Schoolnik, Gk
Published in
Proceedings of the National Academy of Sciences
The rugose colony variant of Vibrio cholerae O1, biotype El Tor, is shown to produce an exopolysaccharide, EPSETr, that confers chlorine resistance and biofilm-forming capacity. EPSETr production requires a chromosomal locus, vps, that contains sequences homologous to carbohydrate biosynthesis genes of other bacterial species. Mutations within this...
Wenz, Hm Lindsay Hinck Cannon, P Navre, M Ringold, Gm
Published in
Proceedings of the National Academy of Sciences
We have recently characterized an adipocyte cDNA (clone 5) that is enhanced in expression by environmental and hormonal conditions favoring adipogenic differentiation. Moreover, certain agents including fibroblast growth factor and phorbol 12-myristate 13-acetate (but not epidermal growth factor) markedly inhibit clone 5 gene expression and prevent...
Korostelev, Andrei Laurberg, Martin Noller, Harry F
Published in
Proceedings of the National Academy of Sciences of the United States of America
A macromolecular X-ray crystal structure is usually represented as a single static model with a single set of temperature factors representing a simple approximation of motion and disorder of the structure. Multiconformer representations of small proteins have been shown to better describe anisotropic motion and disorder and improve the quality of ...
Hüttenhofer, A Harry Noller
Published in
Proceedings of the National Academy of Sciences
Hydroxyl radical is a useful probe of the accessibility of the sugar moiety of nucleic acids to solvent. Here we compare the accessibility of free and ribosome-bound yeast tRNA(Phe), Escherichia coli tRNA(Phe), and E. coli tRNA(Leu2) to attack by hydroxyl radicals generated from Fe(2+)-EDTA. When bound to the P site of 30S ribosomal subunits, a dis...
Sutton, Ca Ares M, Jr Hallberg, Rl
Published in
Proceedings of the National Academy of Sciences
Two cycloheximide-resistant mutants of Tetrahymena thermophila were analyzed to determine the site of their cycloheximide resistance. The mutations in both strains had been previously shown to be genetically dominant and located at separate loci (denoted Chx-A and Chx-B). Strains carrying these mutations were readily distinguished by the extent to ...
Saxton, Wm Porter, Me Cohn, Sa Scholey, Jm Raff, Ec Mcintosh, Jr
Published in
Proceedings of the National Academy of Sciences
Preparations of kinesin, a microtubule-based force-producing protein, have been isolated from Drosophila melanogaster embryos by incubation of microtubules with a nonhydrolyzable ATP analogue and gel filtration of proteins released from the microtubules by ATP. These preparations induced MgATP-dependent microtubule gliding in vitro with a Km for Mg...
Chamberlain, Cp Waldbauer, Jr Fox-Dobbs, K Newsome, Sd Koch, Pl Don Smith Church, Me Chamberlain, Sd Sorenson, Kj Risebrough, R
...
Published in
Proceedings of the National Academy of Sciences
We used carbon and nitrogen isotopes to investigate changes in the diet of California condors from the Pleistocene to the recent. During the Pleistocene, condors from California fed on both terrestrial megafauna and marine mammals. Early accounts reported condors feeding on the carcasses of marine mammals, but by the late 1700s, condor diets had sh...
Jevgenij Raskatov Nickols, Ng Hargrove, Ae Marinov, Gk Wold, B Dervan, Pb
Published in
Proceedings of the National Academy of Sciences
Gene regulation by DNA binding small molecules could have important therapeutic applications. This study reports the investigation of a DNA-binding pyrrole-imidazole polyamide targeted to bind the DNA sequence 5 -WGGWWW-3 with reference to its potency in a subcutaneous xenograft tumor model. The molecule is capable of trafficking to the tumor site...
